Anderson's career at Manchester United appears all but over after the Brazil international was left out of Louis van Gaal's squad for the club's pre-season tour to the United States.
The 27 year old joined Fiorentina on loan in January but made just five starts for the Serie A club and returned to Old Trafford this summer ahead of the new campaign.
But despite Van Gaal being keen to evaluate his United squad, having only officially begun his tenure this week, the omission of Anderson from the club's pre-season tour signals the end of his seven-year career at the 20-time English champions.
Signed by Sir Alex Ferguson from FC Porto in 2007, Anderson has failed to endear himself to United fans during his spell in the Premier League while also being unable to hold down a regular first-team place.
Though the signing of Ander Herrera from Atletico Madrid has already increased competition in midfield, Van Gaal was set to have an open mind regarding his squad with just a month before the season starts but the side-lining of Anderson suggests his career lies away from United.
Robin van Persie, Javier Hernandez, Patrice Evra, Adnan Januzaj and Marouane Fellaini, whose countries all reached the knock-out stages of the World Cup are rested from the tour while Nick Powell and James Wilson – who have been tipped for future first team roles – have also been left out.
Ben Amos, Sam Johnstone, Michael Keane, Wilfried Zaha, Jesse Lingard and Will Keane, who all had spells out on loan last term will get the opportunity to impress, as will new signings Luke Shaw and Spaniard Herrera.
Darren Fletcher's return from surgery continues with a call up to the squad while the likes of David de Gea, Juan Mata, Wayne Rooney and Shinji Kagawa, who all played in the World Cup, are included.
Michael Carrick is the only absentee due to injury after undergoing ankle surgery which will rule him out for 10-12 weeks.
United face four matches on their United States tour, starting against LA Galaxy on 21 July before games against Serie A duo Roma and Inter Milan and European Champions Real Madrid.
Manchester United Tour squad: De Gea, Lindegaard, Amos, Johnstone, Rafael, Evans, Smalling, Jones, Shaw, Blackett, M Keane, James, Herrera, Cleverley, Fletcher, Young, Zaha, Kagawa, Mata, Valencia, Nani, Lingard, Welbeck, Rooney, W Keane.