Veteran BBC broadcaster Dave Lee Travis denies a charge of indecent assault against a woman, aged over 16, in January 1995, a court has heard.
Travis, 68, entered his not-guilty plea at Westminster Magistrates' Court in London and was told to appear for a further hearing at Southwark Crown Court on 8 May.
Police charged him after receiving authorisation by the Crown Prosecution Service last month.
It comes after he was acquitted of 12 counts of indecent assault following a trial at Southwark Crown Court in February.
Travis, who was charged under his real name of David Griffin, denied all the charges and described the accusations against him as "outright lies" from women who could "smell money".
He faces a retrial after the jury failed to reach a decision on two outstanding charges of indecent assault and sexual assault.
Speaking outside the court following the news of his retrial, Travis said: "The nightmare is now going to go on. All I can say is, this whole thing started when I was 67 and I just hope it will end by the time I'm 80."