England will be without Brad Barritt, Tom Croft and Henry Thomas for the remainder of the Six Nations Championship after the trio withdrew from the squad ahead of the match against Scotland.
Barritt suffered an ankle sprain in Saracens' win over Wasps while Croft and Thomas are out with respective shoulder problems picked up while on club duty for Leicester and Bath.
The group become the latest additions to a lengthy England injury list which already includes the likes of Joe Launchbury, Manu Tuilagi and Owen Farrell.
Exeter Chiefs fly-half Henry Slade has been called in to Stuart Lancaster's squad to bolster his options at number 10 ahead of the final two games against Scotland on 14 March and France on 21 March.
Geoff Parling and Courtney Lawes are both in contention to figure upon the resumption of the Championship following the harrowing loss to Ireland which derailed England's grand slam ambitions.
"It's unfortunate that Brad was injured but Henry has been playing well for Exeter and it's good to be able to call him in," coach Lancaster said. "We also wish Tom and Henry all the best for their recovery."
There is some positive injury news for England as Mike Brown continues to recover from suffering a concussion during the thrashing of Italy which forced him to miss the trip to Dublin while Luther Burrell and Kieran Brookes are also recovering as Sam Burgess joins the training squad.
"Mike trained fully last Friday while Luther and Brookes should be fit to train tomorrow," Lancaster added. "We are looking forward to two full days of training before we announce the side on Wednesday.
"Sam Burgess will also come into camp to further his experience of this environment as Bath have no game at the weekend."