Boris Johnson and Sadiq Khan went head-to-head for the Leave and Remain campaigns respectively in front of 6,000 spectators at the BBC's Big Debate on Tuesday night.
The former mayor of London said Thursday could be the UK's "independence day" while Khan dismissed the Leave campaign as built on lies.
With just two days until the EU referendum it was each side's final attempt to sway undecided voters over whether the UK is either better off in or out of the EU.
Leave relied on Johnson, Labour MP Gisela Stuart and energy minister Andrea Leadsom while Khan, Scottish Conservative leader Ruth Davidson, and Frances O'Grady, general secretary of the Trades Union Congress, argued for case for Remain.
That's all folks. Follow IBTimes UK for all the news and reaction for the BBC Big Debate.
Labour MP and Vote Leave campaigner John Mann has told IBTimes UK that the UK's major trade unions were backing Remain out of "loyalty to what they perceive to be the TUC position".
"What [Unite chief] Len McCluskey did yesterday was catch up with the reality of what's happened within the undercutting of wages with mass migration.
"Tony Blair said to us 30,000 to 40,000 will come from Eastern Europe, he was wrong."
Asked how Labour, which is officially backing Remain, would reconcile itself with its voters who back Leave on 23 June after the referendum, he added:
"We need to see what the results are – we will see that from constituencies. If it is that Labour heartlands have voted Leave and it remains Remain, Labour has to move very, very quickly."
Government tsar and Remain supporter Lord Sugar seems to have put his foot in it with a tweet about Labour MP Gisela Stuart:
Tim Farron: I'm Remain's Jamie Vardy
Speaking to IBTimes UK, Liberal Democrat leader Tim Farron urged UK voters to not blame migrants. "First of all, public services are funded by the British government, the British government is funded by taxes and we know EU migrants contribute £20bn to our economy."
"So if our government is not spending their taxes properly on schools and health, stop blaming migrants for things that are the Toy government's fault.
"Secondly, I can be really flippant but blunt, I disagree with Ruth Davidson – I think Vote Leave do have a plan to tackle migration. It's to take us out of the EU, crash the economy and no one will want to come here."
Asked whether he felt frustrated that he hadn't had a bigger role in the Remain campaign ahead of the 23 June vote.
"I'm a team player and I'm confident that if I'd been on the stage I would have played a blinder, but I had to play the Jamie Vardy role and stay on the bench and score a couple of headers."
Handbags in the Spin Room!
Will Straw, the executive director of Britain Stronger In Europe and son of former foreign secretary Jack Straw, has clashed with Leave's Michael Gove.
Straw claimed his opponent was part of "project hate" and called "lies" claims that Turkey would join the EU and Britain sent £350m a week to Brussels.
Labour MP Chuka Umunna is now on the BBC. He told the broadcaster:
"[On Turkey membership] You have to have all 28 members agreeing and I don't see that happening.
"The idea you deal with migration by crashing economy is ludicrous proposition.
"Leave has absolutely no plan really on what they want to do re. immigration."
Speaking on the BBC, Michael Gove said the campaign "absolutely did not" go over the top.
He goes on to say we cannot control migration in the EU and says Leave is investigating how a former BNP member contributed £600,000 to the campaign.
Gove adds Leave peddled "hopeful and optimistic" arguments
Looks like journalists in the Spin Room didn't hear much of those closing remarks:
I have not heard enough to be confident they have the answers.
The EU is not perfect but benefits far outweigh costs.
We refuse to dismiss experts.
You are better off in. There is nothing more positive than a strong economy supporting jobs.
There is a clear choice between fear and hope.
They say we can't do, we say we can. They say we have no choice but to bow down to Brussels.
Thursday can be our country's 'Independence Day'.
Ruth Davidson "demolishes" Leave security arguments...says Remain:
"The other side have said throughout this debate that they don't like experts but when it comes to keeping our country safe and secure I want to listen to the experts. So when the head of GCHQ says we are safer in the EU I listen.
"When five former NATO chiefs say we are safer in the EU I listen. When the head of Europol who is a Brit says we are safer in the EU I listen.
"When the head of MI5 and MI6 says we are safer in the EU I listen. When all of our major allies, Canada, America, Australia and New Zealand, say we are safer in the EU I listen.
"And if it comes to a choice to listening to all of these people, even if they are experts or listening to these three about who keeps my family safe, I'm going to vote for them every single day of the week and twice on a Sunday. Don't risk it."
Leave campaign argues 60% of laws in the UK are made by the EU.
The House of Commons library actually says it has made between 13% and 65% of UK law.
Of course we cooperate with the EU and we will continue to do so
UK security rests on Nato cooperation, not the EU
Leadership of the Americans brought post-war peace, not the EU
We have one last chance to keep our democracy and make sure in the future you can sack government
We should be building bridges not walls
EU is wrong to evolve into United States of Europe
EU was mandated to resolve Balkans crisis - about one million people died
EU telling home secretary she cannot deport serious criminals
Allies are saying we are safer together
They other side has lost debate on economy...so it's lies and scaremongering
We can veto any plans for closer union
Why are you a quitter [to Stuart]?
There is a danger in remaining
All of those people currently charged with our protection say we are safer in the EU
On sovereignty and democracy...
If we chose to remain we are part of single market with one set of rules we help make
You are not less British if you are in the EU
EU has utterly outgrown its roots
There is a failing currency and they have to sort their problems
Do not be this inward trading block
We will become increasingly insignificant with 28 members, plus more to come
We have vetoes over nations joining...we lose control by not being at the table
60% of rules and regulations come from the EU
The truth is there are five presidents of the EU and can anyone name them? You can't vote for them and you can't kick them out
Where you have a fantastic market, then you must have fair rules you have to play by
I'll be honest - I'm proud of being British but there is nothing about putting jobs at risk
We have heard enough of project fear over the last couple of weeks
European Court of Justice is acquiring more power...it adjudicates in divorce laws, nothing to do with the single market
Caroline Lucas, Green Party (Remain)
You are more likely to be treated by an EU national working in the NHS
Migration goes two ways live, study and work in 28 countries ourselves
Lets ringfence migrants' taxes and spend them on new leisure centres and libraries
The question was what migration numbers should be...
We need to look at the skills we want to bring in here
We should not discount the contribution migrants make
My big issue is you don't fund schools and hospitals and control migration by crashing the economy
We would have a £10bn dividend to spend on priorities if we left the EU
This country will do incredibly well if we take back control
Migration is a concern for many people but leaving the EU is not the answer
How is having more control if there is double the immigration under the Aussie-style points system?
Jeremy Corbyn said there was no way of controlling immigration if we are in the EU
I think the Leave campaign is selling a big con - they are pretending to reduce the numbers [of migrants]
More from the Spin Room:
"Sadiq Khan, the Mayor of London, says there's no chance of Turkey joining the EU and accuses Vote Leave of "scaremongering" over the issue.
"But Boris Johnson points out that it's the government policy to support Turkey's accession to the 28-nation-bloc.
"Meanwhile, Ruth Davidson quotes an LBC Radio interview of Johnson two months ago, when the Vote Leave campaigner apparently said "it was not on the cards".
"David Cameron, who's not in attendance, has previously stressed that the UK would have a veto on the question of Turkey joining the EU."
Next up, immigration:
The first thing we should do is celebrate immigration.
We need to look at numbers and pressure large scale immigration is causing on NHS, wages.
We want Aussie-style points system so we get workers for all our services.
184,000 net from the EU, 77,000 without a job - it is obvious we need to take back control.
Of course we need immigration policy.
You won't hear me say this often but David Cameron deserves credit for the deal he secured with the EU - you can't take benefits until you pay tax.
Boris, you are scaring people to vote Leave. You are telling lies. You used taxpayer money to say Turkey is joining the EU.
Turkey is not about to join - that is a big fat lie. At this rate it will take year 3000 for Turkey to accede.
I am an immigrant, mother and grandmother - if we have uncontrolled immigration we will have situations like 50,000 missing school places in West Midlands.
We are spending £1.9bn of your money to speed up accession of Turkey.
There would be staffing crisis if we lost thousands of EU/non-EU workers from the NHS.
Free movement does not allow us to even ask if people have criminal records - it is creating enormous problems.
Uncontrolled immigration means we discriminate against 92% of the world.
We are required to allow people to come here from the EU whether they are trained or not.
Leave does not have alternative plan. They want points system but Migration Watch says that will lead to higher migration.
Turkey accession is not on the cards - that's what Boris said two months ago.
Tim Farron, Lib Dem leader:
9/10 experts say we should remain
Michael Gove said we had enough of experts perhaps that explains why he was such a dreadful education secretary - when I take my children to school I want them to be taught by experts, not the man from the pub.
Tim Martin, chairman of Wetherspoons: Apart from John Lewis, no one pays a bigger bonuses than us.
Justin King, former Sainsbury's boss: We are going to see prices go up, poorer economy if we leave.
More than 250 tweets have been sent between the Stronger In and Leave campaign Twitter accounts in 30 minutes...
Second question is about employment and social rights:
Can Leave promise us today that you will protect each and every right we won through EU?
Euro is a broken promise - you are going to pay for bailout after bailout.
The worst thing for workers is businesses going out of business.
The Eurozone is growing faster than our economy and that of the US.
Size matters - bigger the size the more clout you have.
If you are company in US or China you can have offices in London and have gateway to 500m customers in EU.
The EU is, I'm afraid, is a job destroying machine.
Tata Steel - we are told we cannot cut energy costs to protect Port Talbot because Brussels says no.
We cannot export haggis to America!
Leave won't tell us how many jobs will go and what will replace single market.
If we come out we go back to the queue.
80% of world's economy is not in the single market.
Many countries do more business with the EU than the UK does.
28 member states cannot sort a takeaway countries.
Some early analysis from the Spin Room by Ian Silvera:
"Boris Johnson, the chief Vote Leave campaigner and former Mayor of London, attempted to outflank the Remain team from the left.
"The top Conservative said he was unhappy with the UK's current pay differentials between FTSE 100 chiefs and employees.
"His argument isn't one too different to ones made by Frances O'Grady, the general secretary of the Trades Union Congress and Remain panellist."
We are spending billions are year that go into a big black hole.
The EU has done a terrible job negotiating trade deals for us.
All the experts say leaving the EU would cause problems.
What is your plan? How would you make sure terms of trade are better than they are now?