Garbine Muguruza
Garbine Muguruza saw off challenge of world no. 1 Serena Williams.GettyImages

Day four of the French Open produced what will comfortably go down as the shock of the competition after defending champion Serena Williams suffered elimination in the second round.

While Novak Djokovic and Rodger Federer recorded routine victories in their Roland Garros campaigns, Wednesday saw 20-year-old Garbine Muguruza send tremors shaking through the women's draw with a 6-2 6-2 win over the defending champion.

It was a remarkable result for so many reasons. The world no. 1 cut a frustrated figure as the young pretender eased to victory as she maintained her aggressive control and struck the ball superb en route to consigning Williams to her worst ever score line in her 16 year grand slam career.

And following Li Na's first round defeat to Kristina Mladenovic of France, the American's exit also marks the first occasion in grand slam history where the top two seeds in the women's draw have been eliminated from competition before the third round.

It was a miserable day all round for the Williams family. At the start of the morning it appeared a Venus v Serena showdown was on the cards in the third round but a 2-6 6-3 6-4 defeat to Slovakian teenager Anna Schmiedlova brought the elder sister's campaign in the French capital to an early end.

The seven time grand slam champion took the opening set and followed up with an early break in the second but the 19-year-old responded in scintillating fashion, taking eight of nine games to eliminate 29th seeded Venus.

In the men's singles, Djokovic made light work of Jeremy Chardy, easing past the Frenchman 6-1 6-4 6-2 while Roger Federer sealed his place in the next round with an equally comfortable 6-3 6-4 6-4 win over Diego Sebastian Schwartzman.

The Swiss will play Russian 31st seed Dmitry Tursunov in the next round for a place in the last 16 while Djokovic awaits a meeting with 25th seed Marin Cilic of Croatia

They were joined by Tomas Berdych, who recovered from an early scare to beat Kazakhstan's Aleksandr Nedovyesov 6-7 (4-7) 6-4 7-5 6-3 and Jo-Wilfried Tsonga who defeated Jurgen Melzer 6-2 6-3 6-4.