Andy Murray will begin his French Open campaign against a qualifier yet to be determined in his first round match. Joanna Konta meanwhile has been handed a tough opening draw against Julia Goerges of Germany.
Elsewhere in the draw, world No1 Novak Djokovic has been drawn against South Korea's Yen-Hsun Lu in his opener, while Stan Wawrinka will begin the defence of his crown against Lukas Rosol, currently ranked 68th in the world. Rafael Nadal meanwhile will begin his campaign against huge-serving 28-year-old Australian Sam Groth.
Murray can face Japan's Kei Nishikori in the quarter-finals and could meet 2015 champion Wawrinka in the semis, should they progress that far. Nadal meanwhile is lined up to face France's Jo-Wilfried Tsonga in the last eight stage and could take on Djokovic in the semis.
British hopeful Kyle Edmund will also begin his campaign against a qualifier and could face John Isner in the second round should he progress. Fellow Brit Aljaz Bedene will also face a qualifer in his round one match.
In the women's draw, world No1 Serena Williams, a two-time champion at Roland Garros, will take on 76th ranked Magdalena Rybarikova in her opening match. British No1 Joanna Konta will begin her campaign against the experienced Julia Goerges of Germany.
Laura Robson meanwhile will take on Andrea Petkovic in her first round match after returning from a wrist injury, while Heather Watson was drawn against Nicole Gibbs. Noami Broady will take on Coco Vandeweghe.
The draw followed news that Gael Monfils has become the latest to pull out of the second grand slam of the year. The 29-year-old Frenchman has been suffering from a viral infection and has been advised by doctors to withdraw from competition.
The 17-time grand slam winner Roger Federer also withdrew from Roland Garros this week, explaining he did not want to take any unnecessary risks with a back injury he is carrying.