England sprinter Dwain Chambers and Wales boxing gold medal hopeful Fred Evans are the latest high-profile athletes to withdraw from the Commonwealth Games in Glasgow.
Chambers was due to compete at a third Commonwealth Games as part of the 4x100m team but has withdrawn to focus on next month's European Athletics Championships in Zurich. Team England will not replace the former world indoor champion.
Meanwhile Evans, among Wales' best medal hopes having claimed boxing silver at the London 2012 Olympics, has been denied accreditation following checks from Home Office and Games officials.
The 23 year old Evans was in April forced to pay £1,228 in fines and costs after admitting his part in a nightclub assault in Birmingham.
Wales team boss Brian Davies said: "The key Welsh and British sporting agencies have been supportive in our attempts to get Fred to the Games and we are bitterly disappointed at the outcome.
"Fred is a world-class boxer and has handled this difficult situation in a very disciplined manner - we thank him for his professionalism and wish him all the best for his future ambitions."
Glasgow 2014 chief executive, David Grevemberg, added: "Every athlete is subject to the background checks that are conducted throughout the accreditation process.
"That's made very clear up front and it was exactly the same process that was used in London 2012. We need to comply with UK-wide regulations.
"I think the nature of the Commonwealth Games is that this accreditation process starts in March and the team selections are roughly not until six weeks before the Games."
While Chambers, 36, joins the injured England heptathlete Katarina Johnson-Thompson in pulling out of on the eve of the Games, Evans becomes the latest in a long-line of Welsh medal hopes to withdraw.
World cycling champion Becky James, world triathlon gold medallists Helen Jenkins and Non Stanford as well as expelled 800m runner Gareth Warburton – who has been charged over anti-doping violations - are among the other athletes who will not compete.
Jamaican 100m sprint pair Yohan Blake and Asafa Powell, Olympic heptathlon champion Jessica Ennis-Hill and cyclist Mark Cavendish are the other high-profile absentees from Glasgow.