Kell Brook faces the second defence of his IBF welterweight crown against Frankie Gavin in an all-British bout at the O2 Arena on 30 May.
Brook overcame mandatory challenger Jo Jo Dan in March and faces the another challenge to his title in the form of former world amateur champion Gavin, after the Birmingham-born fighter turned down an offer to face Timothy Bradley in a world title eliminator.
The fight leaves the winner on a collision course for a winter meeting with Amir Khan, who has not yet confirmed his next opponent after backtracking on a pledge to face Chris Algieri.
"I never expected to be out again so quickly but I can't wait," Brook said. "I respect Frankie for stepping up, he has called for this fight for a long time and after he was given a final eliminator against Tim Bradley it made sense for him to take the challenge.
"I feel like I'm unbeatable right now and Gavin isn't going to be able to cope with my speed and power on fight night. The O2 is one of the best arenas in the world and it's an honour to headline a card like this."
Gavin, defeated once in 23 career fights, added: "These are the fights you want to be part of and are where I belong. I've dreamt of a world title and I will be 100% ready physically and mentally for Kell Brook.
"Of course it's going to be tough but I believe Kell is beatable and I've always felt that I have got the skills and the team to cause an upset."
The stellar undercard for the Greenwich show includes London 2012 Olympic heavyweight champion Anthony Joshua facing Kevin Johnson, four months on from the Briton pulling out of a clash after suffering a stress fracture in his back.
Kevin Mitchell and Lee Selby also feature in two of three world title fights, against Jorge Linares and Evgeny Gradovich respectively, in an event which will be available via Sky Sports Box Office.