Steven de Jongh has become the latest victim of Team Sky's anti-doing stance ending his three-year spell as sports director.
Following the damming allegations surrounding Lance Armstrong's doping past, which saw him stripped of his seven Tour de France wins, general manager David Brailsford said riders and staff would be interviewed and asked to declare their doping history.
American Bobby Julich departed last week after admitting to using EPO during his racing career and De Jongh becomes the latest member of the squad's staff to leave as Team Sky's ruthless position over previous doping offences continues.
"There's no doubt about Steven's work with us or his approach," Brailsford said. "He's been a highly-valued Sports Director and colleague over three seasons.
"Steven deserves our respect for the courage he's shown in being honest about the past and it's right that we do our best to support him.
"He has our best wishes for the next step in his career."
Meanwhile, Sean Yates, Team Sky's lead sports director has confirmed his retirement from cycling after three decades in the sport.
Yates, among those who were interviewed concerning their doping history, made no admissions during the process and whose departure is motivated by personal reasons.
"Sean joined us in our first year and has been with us for three tough but rewarding seasons," Brailsford added.
"After a long career in professional cycling, he has told us that he wants to move on, for purely personal reasons.
"Sean has been a great support to the riders on the road and a valuable colleague to us all. We wish him the best for the next step in his life."
The 52 year old Yates played a pivotal role in Bradley Wiggins becoming Britain's first ever winner of the Tour de France earlier this year.
Brailsford and sports psychiatrist Dr Steve Peters will continue to interview all 80 members of the Team Sky squad, each of whom have been asked to sign a declaration stating they have never doped, with additional departures expected following the revelations of Julich and De Jongh.
As a result of Armstrong's results being erased from the records, Wiggins has be given third place in the 2009 Tour de France, the International Cycling Union has confirmed.