Uncapped trio Semesa Rokoduguni, George Kruis and Calum Clark have been included in England's 33-man squad for the QBE internationals in November.
Rokoduguni has scored 11 tries in 23 starts for Bath and his impressive form has earned him a maiden call-up alongside Saracens' Kruis and Clark, of Northampton Saints.
England face New Zealand, South Africa, Samoa and Australia in four tests next month less than a year out from the World Cup on home turf.
The squad, again captained by Chris Robshaw, includes tight-head Kieran Brookes, fly-half Stephen Myler, centres Jonathan Joseph and Billy Twelvetrees and winger Marland Yarde in a group reflective of the opening weeks of the domestic rugby season.
Chris Ashton, Danny Cipriani and Freddie Burns are among the notable absentees, while coach Stuart Lancaster is also without a host of players through injury.
Alex Corbisiero, Dan Cole, Tom Croft, Tom Johnson, Geoff Parling, Chris Pennell, Ed Slater, Mako Vunipola and Tom Youngs have all been ruled out for the four autumn fixtures. However Manu Tuilagi could yet be available, despite being ruled out by club Leicester Tigers with a groin injury.
Lancaster said: "The first six rounds of the Premiership and opening round of European action have given us plenty to think about as coaches with a lot of players putting their hand up for selection, and we are excited by the options available to us.
"We are still monitoring a number of players injury-wise and with another intensive round of European games to come, including the Wasps-Harlequins and Sale-Clermont games on Sunday, there are likely to be additions to the squad after the weekend as we move into the training camp and towards the games - so there is plenty to be decided before the New Zealand match comes around.
"We have four quality sides coming to Twickenham for this QBE Internationals series and we are really looking forward to playing at home in front of our passionate supporters as we continue the build up to the World Cup in 12 months' time.
"There are a number of players unavailable for this series because of long-term injury (listed below) but they are all on the road to recovery and, as always, it gives the opportunity for others to come in and stake their claim.
"We have included Jonathan [Joseph] as an injury replacement for Manu. It is obviously disappointing to lose Manu but JJ has been in great form and deserves this chance. Our medics will continue to work closely with Leicester's and ensure that Manu is supported in his rehabilitation from his injury."
England 33-man Senior Squad
Forwards (16): Joe Marler (Harlequins), Matt Mullan (Wasps), Kieran Brookes (Newcastle Falcons), David Wilson (Bath Rugby), Dylan Hartley (Northampton Saints), Rob Webber, Dave Attwood (Bath Rugby), George Kruis (Saracens), Joe Launchbury (Wasps), Courtney Lawes (Northampton Saints), Calum Clark (Northampton Saints), James Haskell (Wasps), Ben Morgan (Gloucester Rugby), Chris Robshaw (Harlequins, captain) , Billy Vunipola (Saracens), Tom Wood (Northampton Saints)
Backs (17): Danny Care (Harlequins), Lee Dickson (Northampton Saints), Ben Youngs (Leicester Tigers), Owen Farrell (Saracens), George Ford (Bath Rugby), Stephen Myler (Northampton Saints), Brad Barritt (Saracens), Luther Burrell (Northampton Saints), Kyle Eastmond (Bath Rugby), Jonathan Joseph (Bath Rugby), Billy Twelvetrees (Gloucester Rugby), Mike Brown (Harlequins), Alex Goode (Saracens), Jonny May (Gloucester Rugby), Jack Nowell (Exeter Chiefs), Semesa Rokoduguni (Bath Rugby), Marland Yarde (Harlequins)