England have announced their 25-man squad that will travel to Rome for the 2016 Six Nations fixture against Italy at the Stadio Olimpico on 14 February. Wasps centre Elliot Daly and Gloucester back row Matt Kvesic have both been released back to their clubs, while young forwards Maro Itoje and Josh Beaumont are retained.
"We'll pick the best 23 to play against Italy and the conditions in Italy will be different, so the squad will be slightly different," head coach Eddie Jones, whose first match in charge ended with a 15-9 win over Scotland at Murrayfield, said earlier on Tuesday. "The playing conditions will be considerably different.
"They will be warmer, a faster pitch, different referee so I am anticipating we will be able to get our attack going a lot quicker than we did against Scotland. I've said it's going to be a faster game so we could pick a faster pack."
Unfancied Italy were unlucky to lose their opener against France, with Sergio Parisse and Carlo Canna crossing the try line before the skipper attracted bemusement by attempting a last-gasp drop goal in a narrow 23-21 defeat in Paris.
Jacques Brunel has yet to name his starting team to face England, but Treviso flanker Robert Barbieri has been drafted into the 30-man squad as cover for the injured Jacopo Sarto.
Wing Andrea Buondonno could win his first cap as David Odiete is expected to be sidelined for a fortnight due to a sprained ankle. Scottish-born Zebre lock George Biagi's involvement will depend on his recovery from a muscular problem sustained at the Stade de France.
England's 25-man squad to face Italy:
Forwards: Dan Cole, Jack Clifford, Jamie George, Dylan Hartley, James Haskell, Paul Hill, George Kruis, Joe Launchbury, Courtney Lawes, Joe Marler, Chris Robshaw , Billy Vunipola, Mako Vunipola, Maro Itoje, Josh Beaumont
Backs: Mike Brown, Danny Care, Ollie Devoto, Owen Farrell, George Ford, Alex Goode, Jonathan Joseph, Jack Nowell, Anthony Watson, Ben Youngs