Darren Wilson did not receive severance pay when he resigned as a Ferguson police officer in the wake of the fatal Michael Brown shooting.
Wilson had been on administrative leave since shooting the unarmed teenager on 9 August and was last week cleared of any criminal wrongdoing by a grand jury.
He resigned five days after the decision, citing safety concerns over his colleagues.
Ferguson mayor James Knowles has announced that Wilson will not receive further pay or benefits following his resignation.
His resignation letter read: "I, Darren Wilson, hereby resign my commission as a police officer with the City of Ferguson effective immediately.
"I have been told that my continued employment may put the residents and police officers of the City of Ferguson at risk, which is a circumstance that I cannot allow.
"For obvious reasons, I wanted to wait until the grand jury made their decision before I officially made my decision to resign.
"It was my hope to continue in police work, but the safety of other police officers and the community are of paramount importance to me.
"It is my hope that my resignation will allow the community to heal. I would like to thank all of my supporters and fellow officers throughout this process."