Laura Robson became the second British exit from the French Open in as many days as the 18-year-old was beaten in straight sets by Spaniard Anabel Medina Garrigues.
After being handed a place in the main draw following injury to Silvia Soler-Espinosa, Robson could not take advantage of her reprieve in her first appearance at the clay-court slam.
The British No.4 was broken in her opening service game by the Medina Garrigues, who with 10 clay titles to her name is a seasoned victor on the surface.
Robson eventually began to find her range with her powerful forehand, forcing break-point opportunities in the fifth game, but the world No.31 stood firm to take the first 6-2.
The experience of the 29-year-old then shone through in the second set as she stifled Robson's direct hitting, and continued to make first serves, surging into a 5-0 lead.
And she quickly repeated her straight sets win over the 18-year-old at last year's US Open second round, winning the second 6-1 to secure passage into the next stage.
Elsewhere in the women's draw on day two at Roland Garros, world No.1 Victoria Azarenka recovered from a set and a double-break down to overcome Italian Alberta Brianti.
The Australian Open champion got off to the ideal start on Philippe Chatrier, breaking her 32-year-old counterpart early in the first set.
However, her fortunes quickly turned on their head when the Belarusian was broken and then lost a first-set tiebreaker 8-6, amassing 28 unforced errors in the process.
Brianti smelt blood and racked off four successive games in the second set, which included two breaks of serve to put herself on the brink of her first ever win at the French Open.
An epic fifth game saw Azarenka save break-point with a second serve ace, and then regain momentum as she saw out six successive games to take the second set 6-4.
It was a blow Brianti wouldn't recover from, as her 22-year-old opponent cruised into the second round, winning 6-2 in the third.