Brendan Rodgers confirmed midfielder Joe Allen and Pedro Chirivella picked up injuries in Liverpool's 2-1 friendly win over Swindon Town.
The Welsh international started in the final pre-season fixture of the summer at the County Ground on 2 August. He picked up a hamstring injury and was forced to withdraw in the 13th minute.
The Northern Irishman revealed Allen will undergo a scan on Monday (3 August) and is set to miss the start of the season after Rodgers admitted the injury doesn't look good for Liverpool. The Merseyside club face Stoke City in the opening fixture of the new campaign.
"I think Joe was his hamstring, unfortunately, so that doesn't look so good for us. But we'll see how that is tomorrow when he has a scan," Rodgers told Liverpool's official website.
Chirivella, the academy midfielder, came on as a second half substitute in the 76th minute to replace Emre Can. The former Swansea manager said the Spaniard has also picked up a late injury in the clash against Swindon.
"Young Pedro looked like he had bent his wrist and it looked a sore one. I'll see how he is in the changing room," he stressed.
The clash against the League One side saw the Reds' two summer signings Christian Benteke and Roberto Firmino make their debut for the Anfield club. The Brazil international had an injury scare in the second minute of the match.
Firmino was clattered by goalkeeper Lawrence Vigouroux, who is on loan from Liverpool at Swindon. However, that did not stop him from continuing on the pitch before being substituted in the second half.
Rodgers ruled out any injury scare for the South American player, but said he picked up a knock.
"Firmino is fine. I just think he overstretched a little bit and took a knock. We are just managing his minutes and I thought he did very well," the Liverpool manager said.
"I was impressed with his positivity. You can see he wants to create when he's on the ball and he works hard. What was pleasing for me is that when we were in and around the box, we looked a threat."