Durham opener and Hampshire all-rounder in contention for selection for final two Tests.
Tourists finish day 268-8 with India yet to bat.
India face England in the third Test at Mohali on 26 November.
Stuart Broad also misses out with a foot problem.
India currently lead the five match Test series against England 1-0.
Kohli led India to a 246-run win against England in the second Test at home.
Opening batsman Gautam Gambhir has been dropped from the 16-man squad for final three matches.
India beat England by 246 runs in the second Test to take a 1-0 lead in the five-match series.
Tourists lose their final eight wickets for 71 en route to a 246-run defeat in Visakhapatnam.
Shastri says Bengal is not limited to just one prince.
India and England are currently in action in the second Test match at Vizag.
Haseeb Hameed and Alastair Cook both fall to leave tourists with an uphill battle on day five.
The ECB's new rule comes ahead of the second death anniversary of Australian batsman Phillip Hughes who was hit by a bouncer.
Hosts lead by 298 runs with Kohli and Ajinkya Rahane not out.
India and England are in action in the second Test at Visakhapatnam.
Tourists trail by by 352 runs on second day in Visakhapatnam.
Kohli and Root are in action in the second Test between India and England at Visakhapatnam.
Hosts closed play at 317-4 with England yet to bat.
The stylish opener looks set to replace Gautam Gambhir, who struggled for runs in Rajkot.
Alastair Cook is reportedly unsure on how long he wants to remain as England Test skipper.
Hameed, 19, scored an eye-catching 82 in England's second innings in Rajkot.
Cook scored his 30th Test century against India at Rajkot.