Billy Twelvetrees will start for England against Australia at Twickenham on Saturday 29 November.
The Gloucester centre replaces Owen Farrell, who has been dropped to the bench after his underwhelming performances against New Zealand, South Africa and Samoa.
George Ford retains his place and will win his sixth cap in Saturday's Test after impressing against Samoa, with Northampton Saints duo Tom Wood and Dylan Hartley coming in for James Haskell and Rob Webber at flanker and hooker respectively.
Saracens fly-half Farrell was moved to inside centre for the win over Samoa in order to accommodate Ford. His relegation to the bench is the first time he has been overlooked for the starting XV since June 2012.
"It's good to be able to give Billy his first start of the series," England head coach Stuart Lancaster said.
"He took on board the feedback we gave him when he first came into camp and he has worked hard both with us and at Gloucester and deserves his chance.
"Owen is a key part of our squad and I am sure he will make a positive contribution should he get the chance."
England team to face Australia: Mike Brown, Anthony Watson, Brad Barritt, Billy Twelvetrees, Jonny May, George Ford, Ben Youngs, Joe Marler, Dylan Hartley, David Wilson, Dave Attwood, Courtney Lawes, Tom Wood, Chris Robshaw (c), Ben Morgan.
Replacements: Rob Webber, Matt Mullan, Kieran Brookes, George Kruis, James Haskell, Richard Wigglesworth, Owen Farrell, Marland Yarde.