The Serbian world number one is eyeing his 18th Grand Slam title with the US Open starting on August 31.
Both Williams and Murray could only make it into the third round of the event.
The Western and Southern Open is being played in New York this year in preparation for the US Open.
The 33-year old Serbian is currently three short of Federer's 20-Grand Slam tally, and two short of Nadal's 19 major titles.
The world number two is the latest star to join a growing list of players who decided to pull out of the US Open.
The 23-time Grand Slam winner hasn't won a major final since the birth of her daughter in 2017.
The USA continues to be the worst-hit country in the world during the novel coronavirus pandemic.
Williams only made it up to the quarter-finals while Gauff was booted out in the semis.
WTA world number one Ashleigh Barty, fifth-ranked Elina Svitolina, and number seven Kiki Bertens have already pulled out of the 2020 US Open.
Players at the Prague Open are advised to stay isolated and not to shake hands with each other during and after their matches.
Nadal is the latest high profile player to pull out of the Grand Slam event.
Spanish health ministers have recommended the cancellation of the event amid the novel coronavirus pandemic.
Kyrgios has always been a critic of returning to competition amid the coronavirus pandemic.
The Duchess of Cambridge is patron of the All England Lawn Tennis and Croquet Club and had earlier visited Bond Primary School in January 2018.
Simona Halep has expressed her concerns about the safety of playing in the Grand Slam.
The Duchess of Cambridge took over Queen Elizabeth's position as royal patron of All England Lawn Tennis and Croquet Club in 2016.
The Adria Tour has been cancelled as the fourth tennis player of the tournament tests positive.
Djokovic will also undergo a novel coronavirus test after other players were found positive.
Players will be undergoing daily temperature checks along with two COVID-19 tests every week during their stay in the USA
Despite winning his match against Zverev, Djokovic bowed out of the tournament
For the dress-up game, the young girls made their sportsman father wear a make-shift tiara and a Scotsman's kilt.
The 38-year old Swiss star is running out of opportunities and his fans are unsure whether he will appear in Wimbledon again.
The newly rescheduled French Open clashes with the timings of several ATP and WTA tournaments
Enrique Iglesias and Anna Kournikova have welcomed their third child, a daughter, after keeping the pregnancy a secret.
Thiem is attempting to secure his first Grand Slam title.
After beating Zverev in the semi-final, Thiem became the first Austrian man to reach the Australian Open final
Rod Laver Arena's roof was closed to combat severe weather conditions
The Serb is aiming to win his 17th Grand Slam title after he stormed into the final, where he will face either Thiem or Zverev
Either Thiem or Zverev will face Federer or Djokovic in the Australian Open 2020 Final
The 16-time Grand Slam winner is aiming to lift his 8th Australian Open title