Wanyama, 25, had been ever-present in Tottenham's starting XI this season but missed the win over Swansea City after taking a knee to the back in the previous game over Burnley.
The midfielder was assessed again ahead of last Saturday's match with Watford but Mauricio Pochettino opted not to risk him, with his side easing to a 4-0 win.
But in an injury update released via the club's official Twitter account, Spurs have confirmed Wanyama is once again available for selection having returned to full first-team training this week.
Back-up goalkeeper Michel Vorm is also hoping to return to the match day squad but will be assessed ahead of the weekend. The Dutchman suffered a knee injury in the 3-1 victory over Swansea on 5 April, a knock that ruled him out last Saturday with 22-year-old Pau Lopez taking his spot on the bench.
Danny Rose meanwhile continues to recover from his knee injury. The 26-year-old had made a strong case of being Tottenham's player of the season before he was struck down during the goalless draw with Sunderland at the end of January, but now stands a chance of returning before the end of the season.
The north London side revealed last week the defender is "on track" to return to first-team training by the end of the month with an update on Thursday stating he is expected to start "outdoor work" next week.
19-year-old defender Cameron Carter-Vickers meanwhile is also being assessed after suffering a knee injury during a training camp with the United States Under-20 side last week.
The powerful centre-half has not played since January but was named in Pochettino's squad for recent games against Burnley and Swansea.
Harry Winks and Erik Lamela, ruled out for the rest of the season with respective ankle and hip injuries, "continue to be monitored."