The likes of Liverpool, Sevilla, Napoli and Celtic will all learn their European fates as they find out who they must beat in order to secure a place in the Champions League group stage.
Where to watch
You can watch the draw unfold live from Uefa's headquarters in Nyon on BT Sport 3 from 11am BST on Friday (4 August). You can also stream the draw live on Uefa's official website.
Some of Europe's top sides will have to deal with the unpredictability of the play-offs as 20 teams will be vying for a spot in the Champions League group stage.
Liverpool are one of only two British teams featuring in the play-offs and will avoid the likes of Sevilla, Napoli, CSKA Moscow and Sporting Lisbon as they look to play in the group stage for the first time since the 2014/15 season.
However, Jurgen Klopp's men could be in for a reunion if they draw Ligue 1 side OGC Nice with former Reds striker Mario Balotelli spearheading the attack for the French team.
As for Brendan Rodgers' Celtic side, they will be looking to make the group stage yet again as they will not meet the likes of Olympiakos, FC Copenhangen, APOEL and Maribor.
Their toughest potential opponents will come in the form of Czech side Slavia Prague.
Seeded: Olympiakos, Celtic, Copenhagen, APOEL, Maribor
Unseeded: Qarabag, Astana, Rijeka, Hapoel Beer-Sheva, Slavia Prague
Seeded teams: Sevilla, Napoli, Liverpool, CSKA Moscow, Sporting Lisbon
Unseeded teams: Steaua Burcharest, Young Boys, Nice, 1899 Hoffenheim, Istanbul Basaksehir
The first leg of the play-offs is scheduled to take place on 14-15 August, with the return games a week later between 22-23 August.
The 10 sides that win their play-off ties will enter the final 32 of the Champions League while the 10 losers will transfer to the Europa League group stage.