Team GB suffered further relay embarrassment as the men's 4x100m team were disqualified after Adam Gemili failed to grab the baton inside the changeover zone.
After trailing the Jamaican team from the outset, the hosts required a simple changeover to Gemili on the final leg to secure a place in Saturday's final.
However, Daniel Talbot failed to find the 18 year old inside the changeover zone, and despite bringing the baton home in 37.90 seconds, the GB team will miss out on a potential medal in the final in disastrous circumstances.
Guilty man Gemili, the world 100m junior champion: "I went off. Maybe I went early or too hard. It's disappointing as we could have been in contention in the final."
Daniel Talbot, who couldn't find Gemili by legitimate means, said: "I don't know what happened. I just couldn't catch Adam. I'm really gutted and it's devastating."
Lead-off man Christian Malcolm added: "We are a team. It's unfortunate. It's one of those things that happens but it's a fantastic performance to run the time we did. Daniel has come in at the last moment. This is a big stadium and it can be overwhelming."
Dwain Chambers, who survived a British Olympic Association appeal to feature at London 2012 said: "At the end of the day we are proud to be here representing the country. We have to build as a team and hopefully we can do better in the future."
The error completes a dismal year for Britain's relay teams, who failed at the European Athletics Championships and the World Junior Championships, while the women's 4x100m team didn't qualify for the Olympics.
There was some joy for the British relay teams on the penultimate day of athletics competition, as the women's 4x400m relay team qualified for the final after finishing third in heat two.
The quartet of Christine Ohuruogu, Eilidh Child, Lee McConnell and Shana Cox helped GB qualify third fastest for the final, a team which could see 400m hurdler Perri Shakes-Drayton introduced.
United States and Russia, who both finished ahead of Britain in heat two were the fastest two qualifiers, with the American's still to bring in 200m champion Alison Felix and 400m gold medallist Sanya Richards-Ross.