Nancy Guthrie missing since January after family dinner
FBI says DNA from glove near Nancy Guthrie’s Tucson home appears to match suspect seen in surveillance video — awaiting confirmation before entering profile into CODIS. Screenshot from YouTube/CNN

The FBI has received preliminary DNA results from a glove found near Nancy Guthrie's Tucson home that could crack open the investigation into the 84-year-old's disappearance two weeks ago.

An FBI spokesperson confirmed on Sunday that one glove with a DNA profile recovered 'is different and appears to match the gloves of the subject seen in the surveillance video'. The agency received preliminary results on Saturday and is now awaiting quality control and official confirmation before entering the unknown male profile into CODIS, the bureau's national DNA database. According to the FBI spokesperson, this process typically takes 24 hours from when the bureau receives DNA.

Glove Matches Masked Intruder

The recovered glove resembles the ones clearly visible in surveillance footage released earlier this week by the FBI. That doorbell camera video, posted by director Kash Patel, shows a masked individual wearing black gloves while tampering with Nancy's front door camera around the time she vanished on 1 February.

In the chilling footage, the person can be seen reaching toward the doorbell with a gloved hand before ripping out a plant from Nancy's yard to block the camera's view. The suspect was also carrying what appears to be a flashlight in their mouth whilst attempting to cover their tracks.

The Pima County Sheriff's Department confirmed that multiple gloves have been recovered during the investigation, with the closest found about two miles from Nancy's home and the furthest discovered up to 10 miles away. It's unclear whether all the gloves are connected to the suspect or if some might be unrelated rubbish.

DNA Could Identify Suspect

If the DNA profile comes back with a match in the criminal database, investigators could have a name within hours. Former FBI supervisory special agent Jim Clemente said that forensic evidence from inside the home is more likely to yield results than outdoor items exposed to Arizona's harsh elements.

Sheriff Chris Nanos has already confirmed that DNA from someone other than Nancy and those close to her was collected from her property, though he would not say where exactly it was found. That genetic material is currently being analysed alongside the gloves at a Florida laboratory.

Suspect Description and Walmart Backpack

The FBI released a detailed description of the suspect on Thursday based on forensic analysis of the doorbell footage. They are looking for a male approximately 5'9" to 5'10" tall with an average build, wearing dark clothing, black gloves, trainers, and a black 25-litre Ozark Trail Hiker Pack backpack.

That specific detail about the backpack could prove crucial. Ozark Trail brand is sold exclusively at Walmart, and law enforcement sources said investigators have already reviewed CCTV footage at one Walmart location in Tucson to track down recent purchases. The FBI will investigate how many of these backpacks were sold and when, according to Joshua Skule, former FBI executive assistant director for intelligence.

Laboratory Dispute Delays Testing

The DNA analysis has been caught up in a row between local and federal authorities over which laboratory should handle the evidence. An FBI source claimed Sheriff Nanos was blocking federal agents from accessing key evidence by sending it to a private Florida lab instead of the FBI's facility in Quantico, Virginia.

Nanos defended his decision in an interview on Friday. 'It adds a step that's not needed', he said, explaining his department has used the same laboratory for years. 'If you have two labs you're looking at, and one's bad or one's better, that's fine. That's not the case here, nobody's saying that.'

Reward and Family Pleas

The bureau has increased its reward to $100,000 (£76,000) for information leading to Nancy's location or the arrest and conviction of anyone involved in her disappearance, double the original $50,000 (£38,000) offer.

Nancy's daughter Savannah, who co-hosts NBC's Today programme, has publicly shared the surveillance images and insisted her family believes their mother is still alive. She and her siblings have made multiple video pleas offering to pay any ransom demanded, though authorities have not confirmed whether ransom notes sent to media outlets are authentic.

Officials have warned that Nancy relies on several medications for her heart condition, heightening concerns about her well-being.

Why DNA Evidence Matters in Cold Cases

The potential DNA breakthrough represents exactly the kind of forensic evidence that has cracked countless investigations, including cases that have gone cold for decades.

'DNA has proven to be a powerful tool in the fight against crime', the department noted in one study. If the recovered glove does contain DNA from Nancy's abductor and that profile matches someone already in the CODIS database, it could significantly accelerate efforts to identify and locate a suspect. The new evidence gives investigators their strongest leads yet in determining who took Nancy Guthrie and where she might be now.